what is the Biochemical Test for the Identification of E. coli?

Morphological characteristics of E. coli

CharacteristicsE. coli
Gram StainingNegative
Shape (Cocci/Diplococci/Rods)Rods
Motility (Motile / Non-Motile)Motile
Capsule (Capsulated/Non-Capsulated)Variable
Spore (Sporing/Non-Sporing)Non-Sporing
Flagella (Flagellated/Non-Flagellated)Flagellated

Biochemical tests for the identification of E. coli

CatalasePositive (+ve)
OxidaseNegative (-ve)
MRPositive (+ve)
VPNegative (-ve)
OF (Oxidative/Fermentative)Fermentative
IndolePositive (+ve)
CitrateNegative (-ve)
UreaseNegative (-ve)
Nitrate ReductionPositive (+ve)
H2SNegative (-ve)
GasPositive (+ve)
GelatinNegative (-ve)
Hemolysis (Alfa/Beta/Gamma)Some Strains shows Hemolysis

Fermentation by E. coli

ArabinosePositive (+ve)
DNaseNegative (-ve)
GlucosePositive (+ve)
InositolNegative (+ve)
LactosePositive (+ve)
MannitolPositive (+ve)
SorbitolPositive (+ve)

Enzymatic Reactions of E. coli

Acetate UtilizationPositive (+ve)
Arginine dehydrolaseVariable
LysinePositive (+ve)
ONPG TestPositive (+ve)
Ornithine decarboxylaseVariable
Phenylalanine deaminaseNegative (-ve)

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