| Characteristics | Enterococcus faecalis |
| Morphological characteristic | |
| Gram Staining | Positive |
| Shape (Cocci/Diplococci/Rods) | Cocci |
| Motility (Motile / Non-Motile) | Non-Motile |
| Spore (Sporing/Non-Sporing) | Non-Sporing |
| Catalase | Negative (-ve) |
| Oxidase | Negative (-ve) |
| VP | Positive (+ve) |
| Indole | Negative (-ve) |
| Citrate | Negative (-ve) |
| Urease | Negative (-ve) |
| Nitrate Reduction | Positive (+ve) |
| H2S | Negative (-ve) |
| PYR | Positive (+ve) |
| Gelatin Hydrolysis | Variable |
| Hemolysis (Alfa/Beta/Gamma) | Variable (Alfa or Beta) |
| Pigment | Negative (-ve) |
| Fermentation | |
| Arabinose | Negative (-ve) |
| Fructose | Positive (D-Fructose) |
| Glucose | Positive (+ve) |
| Hippurate | Positive (+ve) |
| Inulin | Negative (-ve) |
| Lactose | Positive (+ve) |
| Maltose | Positive (+ve) |
| Mannitol | Positive (+ve) |
| Mannose | Positive (D-Mannose) |
| Raffinose | Negative (D-Raffinose) |
| Ribose | Positive (+ve) |
| Sorbitol | Positive (+ve) |
| Sucrose | Positive (+ve) |
| Enzymatic Reactions | |
| Alkaline Phosphatase | Negative(-ve) |
| Arginine dehydrolase | Positive (+ve) |
| Acetoin Production | Positive (+ve) |
| Esculin Hydrolysis | Positive (+ve) |
| Beta Glucuronidase | Negative (-ve) |
| ONPG (β-galactosidase) | Negative (-ve) |
| Pyrrolidonyl arylamidase | Positive (+ve) |
